Job posting will remain open until a suitable candidate is identified.
This is a 100% Limited position for one (1) year with the possibility of extension or conversion to career status.
Position will be filled either as a SRA 1 or SRA 2, based on the qualification and experience of the candidate identified.
The Staff Research Associate will carry out independent research under the direction of the Principal Investigator (PI) on T cell homeostasis, memory T cell formation, and survival in the mouse. Incumbent will manage lab flow cytometry and cell sorting capabilities; run fluorescence-activated cell sort (FACS) and in-vitro assays in the evaluation and analysis of T cell responses; and supervise antibody production, maintenance, and propagation of selected mouse lines based on genotype determination.
Incumbent will also operate and perform standard studies on a FACS for various PIs (Principal Investigators) within the Division of Biological Sciences. Additionally, incumbent will generate data, analyze results, and present results at lab meetings, design new experiments as well as participate in the writing and preparation of scientific manuscripts. This position is also responsible for independently organizing and maintaining the lab, monitoring lab expenditures, and serves as lab radiation, biohazard and safety coordinator. In particular, the incumbent with independently interface with the campus EH&S unit, other labs on the floor and all lab members to regulate appropriate safety guidelines, maintain all scheduling of spaces and any additional regulatory responsibilities. Incumbent will recruit, train, and manage undergraduate research assistants, including overseeing their schedules, and assisting others in their experiments.
Experience using a FACSAria for analysis and cell sorting of lymphocyte population. Particular experience operating fluorescence-activated cell sorters (FACS) such as the BD FacsAria/Fortessa, and conducting physiochemical techniques such as gel filtration, polyacrylamide gel electrophoresis (PAGE), sodium dodecylsuface-PAGE, isoelectric focusing, affinity chromatography, radioimmunoassay, and immunoprecipitation.
Proven experience working with T cells both in vivo and in vitro. Experience with T cell homeosta and memory T cell formation.
Demonstrated experience working with infectious agents of BL2/BL2+ using in vivo models.
Strong theoretical and practical knowledge of biochemistry, molecular biology, immunology and genetics, including relevant laboratory techniques, acquired through a BS in Biology or related field OR an equivalent combination of education and laboratory experience.
Demonstrated experience with sectioning, processing, and analyzing histological samples by confocal microscopy.
Demonstrated experience in sterile technique for the propagation of prokaryotic and mammalian cells.
Extensive knowledge of specialized scientific terminology relating to topics of molecular biology, cell biology and animal physiology.
Experience working with mice as research model system, including experience genotyping, maintaining mouse lines, and performing surgery and dissection.
Proven ability to utilize scientific literature and apply to a research project. Demonstrated experience conducting scientific literature searches, including online databases and other resources.
Proven ability to develop, follow and troubleshoot established lab protocols and lab procedures with direction from PI.
Demonstrated ability to prepare and edit scientific papers, manuscripts, and lab data. Experience presenting research to formal audience.
Excellent interpersonal communication skills to interact well with others to facilitate communication about lab requirements including the ability to discuss and understand day-to-day laboratory affairs, research plans, results and ideas with PI and other lab personnel in a constructive, professional and pleasant manner.
Familiarity with Apple and PC computers and associated word processing and spreadsheet software with a willingness and ability to update and increase skills and knowledge as needed.
Ability to hire and manage undergraduate student employees. Skill to train others in various lab techniques and procedures (safety precautions, preparation of media, dishwashing, equipment usage. laboratory maintenance, etc.).
Knowledge/understanding and at least some experience with Safety Data Sheets, hazard communication and Injury and Illness Prevention Program. Familiarity with a Chemica
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